polyclonal rabbit anti-rat cgrp antibody  (AMRAD Corporation)

Journal: bioRxiv

Article Title: Metastatic infiltration of nervous tissue and periosteal nerve sprouting in multiple myeloma induced bone pain

doi: 10.1101/2022.12.29.522199

Figure Lengend Snippet: (A, B) Effect of anti-netrin-1 treatment (NP137 10 mg/kg, i.p.; dosing days represented by arrows) or vehicle on sham or MM mice. (B) Post-surgical day 26 limb use score of sham or MM mice treated with vehicle or NP137. *p<0.05 by one-way ANOVA followed by Holm-Šídak post-hoc test. (C) Survival plot of NP137 treated mice. (D) Endpoint spleen weight. ****p<0.0001 by one-way ANOVA followed by Holm-Šídak post-hoc test. (E) Effect of systemic NP137 treatment on bone osteolysis, measured as bone volume per total volume (BV/TV). *p<0.05 by one-way ANOVA followed by Holm-Šídak post-hoc test. (F) Representative images of periosteal CGRP + nerve fibres and GFP + MM cells in sham and MM mice treated with vehicle or anti-netrin-1 treatment. (G) Quantification of CGRP + periosteal nerve density. *p<0.05 by one-way ANOVA followed by Holm-Šídak post-hoc test. (H) Number of vehicle- or NP137-treated MM mice presenting 5TGM1-GFP MM cell infiltration to the periosteum. MM= Multiple myeloma. Sham n=3-4; MM n= 5-10.

Article Snippet: Subsequently, slides were placed in vertical chambers (Shandon, Sequenza Immunostaining, Fisher Scientific; #73-310-017), washed, blocked (3% normal donkey serum, 0.3% triton X-100 in PBS) for 2 h RT and incubated o/n with a primary antibody cocktail containing anti-GFP antibody (polyclonal chicken anti-GFP, 1:2000; #A10262, AB_2534023; Thermo Fisher Scientific, Rockford, IL, USA), anti-TH antibody (TH polyclonal rabbit anti-mouse; 1:1000, Millipore; #AB152, AB_390204), and an antibody against calcitonin gene-related peptide (CGRP; polyclonal rabbit anti-rat, 1:5000; #C8198, AB_259091; Sigma-Aldrich, St. Louis, MO, USA) or growth associated protein-43 (GAP43; rabbit anti-GAP43; 1:1000; #AB5220, AB_2107282; Millipore, Billerica, MA, USA).

Techniques: